COMPLETE SCHOOL PROJECT TOPICS & MATERIALS :
CHAPTERS: Chapter 1-5
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ABSTRACT
Burukutu samples were randomly purchased from different shops at Elele market, Army Barracks, Alimini,River state and Obinze market,Army Barracks, Owerri,Imo state, Nigeria. A total of 30 samples were aseptically collected and compositely screened for total aerobic plate count, total coliform count, total fecal coliform count and total fungal count using Nutrient agar (NA), MacConkey(MCA), Eosin methylene blue (EMB) and Sabouraud Dextrose Agar (SDA) respectively by pour plating technique. The microorganisms isolated belong to the genera Escherichia, Staphylococcus, Bacillus, Lactobacillus, Streptococcus, Aspergillus, Rhizopus and Saccharomyces. The Total aerobic plate count of the samples from Elele and Obinze market were 6.20±0.26 cfu/ml and 5.85±0.26 cfu/ml respectively. Total fecal coliform count of the samples from Elele market was 5.10±0.10 cfu/ml, while that from Obinze market was 5.06±0.23cfu/ml. The total coliform count and total fungal count of the samples from Elele market were 5.80±0.08 and 6.20±0.22cfu/ml, while samples from Obinze market recorded 5.50±0.17 and 6.08±0.08cfu/ml. Staphylococcus aureus was the highest occurring bacteria at 27.27% while Sacccharomycescerevisiae was the highest occurring fungi at 40.91%. Statistical analysis showed that there was no significant difference in the microbial counts of the samples collected from Elele market and Obinze market at p-value > 0.05.
CHAPTER ONE
1.0 INTRODUCTION
1.1 BACKGROUND OF THE STUDY
“Burukutu” is a Nigeria indigenous fermented drink. The preparation of this fermented beverage is from guinea corn (Sorghum vulgare) is carried out by villagers in many parts of the world.
Kaffir and Banti beers are similar products produced in south and east Africa respectively. Similar beverages are “Merisa” Sudan); “Bouza (Ethiopia); “Pombe” (east Africa); “Mowa” (Malawi) (Vander Walt, 2003).“Burukutu” commonly known as ‘BKT’ is produced from fermented malted guinea corn (Sorghum vulgare). Other cereals such a maize (Zea mays) could also be used. It is a popular alcoholic beverages of a vinegar-like flavour consumed mostly in the northern guinea savanna region of Nigeria in the republic of Benin and Ghana (Faparusi et al, 2006).Ekundayo (2002) reported that the beverage is sometimes generally made from a mixture of guinea corn and maize.Sorghum malt is rich in amylase particularly -amylase, although the ungerminated grains does not contain β-amylase as in the case of barley. In the republic of South Africa, the brewery of kaffir beer has developed into a major industry in the hand of the authorities with an output comparable in volume to the South Africa output of European beers (Novellie, 2004).
Additionally, a large amount of home brewed Kaffir beer is made in South Africa from commercially malted Sorghum vulgare. the production of the traditional Kaffir beers of south Africa are described by Vander Walt (2003) and Malawi Webb (2001).
The social and nutritional significance of these beverages are considered by Vander Walt (2003).
“Burukutu” is pure and natural drink, that is why after taking even a whole pot of it, one never suffer from any hangover the next morning rather, one is stronger and more active. (adam,1985).“Burukutu” serves as drink and also as food at the same time. Furthermore, “Burukuru” is a beverage of vinegar-like flavour, a brown coloured suspension produced mainly from the grains of guinea corn of the species (Sorghum vulgare) and (Sorghum bicolor) ( Kolawoleet al,2007).it constitutes a major source of energy and protein for people in Asia and Africa and it serves as a staple food for many of the world’s poorest and least privileged people(Hulse et al , 1980).sorghum proteins are less digestible and mineral available than those of the other cereals(Klopfenstein and Hoseney, 1995 “Burukutu” is traditionally prepared form the grains of sorghum in five basic stages or phases which are steeping, malting (germination), mashing, fermentation and maturation.
1.2 AIM OF THE STUDY
The aim of this work is to isolate and characterize, fungi associated with the fermentation of guinea corn (Sorghum vulgare) in“Burukutu” production.
1.3 STATEMENT OF THE PROBLEM
Fermentation takes 2 days in “Burukutu” production. This means a lot for an industrial process. the time lost in the fermentation of the sorghum grain to “Burukutu” production gives a concern. The fungi that causes the fermentation if well identified and stored can be used to inoculated the solution of sorghum grains thereby enhance fermentation. The problem confronting the study is carry out an isolation and characterization of fungi associated with burukutu production with a case study of burukutu production in southern Nigeria.
1.4 SIGNIFICANCE OF THE STUDY
The study is very important in the sense that it will let people to know how “Burukutu” production is carried out.Also, to know the exact fungi used in the production of“Burukutu”.
1,5 SCOPE OF THE STUDY
The study focuses on the isolation and characterization of fungi associated with burukutu production with a case study of burukutu production in southern Nigeria.
1,6 LIMITATION OF THE STUDY
The study was confronted with both logistics and geographical factors thereby restricting the case study
1.7 DEFINITION OF TERMS
BURUKUTU DEFINED
Burukutu is a popular alcoholic beverage of vinegar -like flavor, consumed mainly in the northern region of Nigeria, in the republic of Benin, and in Ghana. It is produced mainly from the grains of guinea corn of the species Sorghum vulgare and Sorghum bicolor
BREWING DEFINED
The process of making beverages is known as brewing. Brewing involves the fermentation of flavored extract derived from the mashing of malted cereals.