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LACK OF QUALITY CONTROL AND SCIENTIFIC EVIDENCE FOR EFFICACY AND SAFETY OF MEDICINAL PLANTS

LACK OF QUALITY CONTROL AND SCIENTIFIC EVIDENCE FOR EFFICACY AND SAFETY OF MEDICINAL PLANTS Abstract This study employed a quantitative survey research design to investigate the historical significance, quality control, and scientific validation of medicinal plants. A structured questionnaire was meticulously designed to gather data from a targeted sample of 120 respondents, offering insights into […]

CHARACTERISING GROWTH BEHAVIOUR OF YEAST STRAINS ISOLATED FROM MANGO FRUIT IN CARBON, NITROGEN AND STRESS ENVIRONMENTS

ABSTRACT The present experiment aims at investigating the growth behaviour of different yeast strains inselected carbon, nitrogen and stress environment to obtain strains with prospects for industrialapplication.   Specifically,   the   study   is   set   to:   isolate   yeast   from   decaying   mango   fruit   anddetermine growth performance of yeast strains in different environments, carbon, nitrogen andstressors. The procedure adopted in […]

PHYSIOCHEMICAL AND SENSORY EVALUATION OF COOKIES PRODUCED FROM BLENDS OF MALTED SORGHUM, SPROUTED BLACK BEANS AND COOKED COCOYAM FLOUR, PROXIMATE ANALYSIS,MINERALS

CHAPTER ONE 1.1 BACKGROUND OF STUDY Over the years the production of cookies of various types has improved with the advancement of new technology in most of the manufacturing industries in Nigeria; the cookies industries tend to be the most popular among most of the manufacturing industries in Nigeria. Cookies vary with types, method of […]

ANTIBACTERIAL ACTIVITY OF HONEY ON STAPHYLOCOCCUS AUREUS ESCHERICHIA COLI AND STREPTOCOCCUS PYOGEN ISOLATED FROM WOUND

ABSTRACT Antibacterial activity of honey obtained from two different locations in Enugu State (Nsukka & Ugwuaji) Nigeria on Staphylococcus aureus, Escherichia coli and Streptococcus pyogens isolated from wound was studied. Agar well diffusion method was used to determine the antibacterial activity of the honey on the test microorganisms. The result revealed that the two honey samples have heavy antibacterial activities against […]

EFFECT OF DIFFERENT CARBON SOURCES ON THE GROWTH OF ANTIMICROBIAL PRODUCING BACILLUS SPECIES IN CITRILLUS VULGARIS

ABSTRACT Comparative examination of three main sources of water supply in Independence layout Enugu was carried out with a view to determine their levels of contamination of bacteria. The sources of water examined are tap water, well water and stream water. The pour plate method was used to examine the water samples. Bacteria isolated from […]

Hepatitis c virus among pregnant women people living with hivaids attending clinic at unth itukuozalla

A total of 50 blood (30 pregnant and 20 HIV) samples were obtained from pregnant women and PLWHA attending clinic at UNTH Ituku-ozalla. Samples were screened for hepatitis c viral infection using the rapid one step hepatitis C virus test strip. Two (6.66%) pregnant women were positive and four (20%) HIV patients were positive for Hepatitis C, giving an […]

PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY OF GRAM NEGATIVE BACTERIA IN THE URINE

ABSTRACT In order to access the prevalence and sensitivity pattern of urinary pathogens, 60 midstream urine samples from students of Caritas University were investigated using cultural methods. Samples were examined microscopically and cultured in blood agar and Macckonkey agar. Disk diffusion method was used for antibiotic testing. Of the 60 urine samples 48 yielded significant […]

SUSCEPTIBILITIES OF SALMONELLA TYPHI AND OTHER BACTERIAL PATHOGENS TO ANTIBIOTICS AND HOT AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA

ABSTRACT The susceptibilities of Salmonella typhi and other pathogens to antibiotics and hot aqueous extract of Hibiscus sabdariffa were investigated using agar diffusion and agar well diffusion methods respectively. Salmonella typhi was sensitive to ampicillin, cetriaxone, ciprofloxacin, gentamycin of ofloxacin and perfloxacin. Nitrofurantoin, ampicillin, clarithomycin and augumentin are resistant. Escherichia coli, Klebsiella spp, and Staphylococcus aureus were sensitive to 50%, 70% and 60% […]

PROXIMATE AND FIBER ANALYSIS OF MULTI-NUTRIENT FEED BLOCK MADE WITH PINEAPPLE PULP AND DIFFERENT BROWSE PLANTS

INTRODUCTION Proximate analysis helps in determination of the major constituents of feed and it is used to assess if a feed is within its normal compositional parameters or somehow being adulterated (Jegede A.V 2015) Goat as Small ruminants animal with four compartment stomach,(Rumen, Reticulum, Omasum and Abomasum) suffer from scarcity in feed supply and pasture […]

DETERMINATION OF DEGRADINGABILITY OF FUNGI ISOLATED FROM HYDROCARBON POLLUTED SOIL ON CRUDE OIL USING GAS CHROMATOGRAPHY

CHAPTER ONE 1.0              INTRODUCTION Crude oils are composed of mixtures of paraffin, alicylic and aromatic hydrocarbons. Microbial communities exposed to hydrocarbons become adapted, exhibiting selective enrichment and genetic changes resulting in increased proportions of hydrocarbon-degrading bacteria and bacterial plasmids encoding hydrocarbon catabolic genes (Leahy and Colwell, 2004). Adapted microbial communities have higher proportions of hydrocarbon degraders […]

PREVALENCE OF GASTROINTESTINAL PARASITES OF CHICKENS

ABSTRACT A survey of gastrointestinal parasites of chickens was carried out in Akure, Ondo state, Nigeria between January and July, 2015. A total of three hundred and twenty seven (327) chickens of different breeds were examined for gastrointestinal infections. Faecal samples obtained from these chickens were prepared for microscopy using flotation technique. The results showed […]

ANTIMICROBIAL AND PHYTOCHEMICAL PROPERTIES OF YOUNGCOCOS NUCIFERA (COCONUT) WATER AND METHALONIC EDXTRACT OF THE YOUNGCOCOS NUCIFERA 9COCONUT) HUSK

CHAPTER ONE 1.0 INTRODUCTION AND LITERATURE REVIEW 1.1 INTRODUCTION Medicinal plants represent a rich source of antimicrobials and many other drugs. The potentials of higher plants as source for new drugs is still largely unexplored. Antibiotic resistance has become a global concern (Westhet al., 2004). The clinical efficacy of many existing antibiotics is being threatened […]

MICROBIAL CONTAMINATION OF YOUGHURT

CHAPTER ONE 1.0   INTRODUCTION  Yoghurt is a fermented dairy product obtained from the lactic acid fermentation of milk. It is one of the most popular fermented milk products in the world and produced commercially at home. (Willey et al., 2008). In its commercial production, non fat or low fat milk is pasteurized cooled to 43°c and are […]

ECONOMIC ASSESSMENT OF SOME METHODS ADOPTED IN YOGHURT PRODUCTION

CHAPTER ONE 1.0 INTRODUCTION Yoghurt is a fermented milk product, produced with a yoghurt starter culture which is a mixed culture of streptococcus thermophilus and lactobacillus bulgaricus in a 1:1 ratio. S thermophilusenjoys a faster growth than L hulgricus.  It adds flavours and aroma to the yoghurt, though both organisms in association produces lactic acid but acetaldehyde and dimethyl propanol, the chief favour component of […]

BACTERIAL COLONIZATION ON HUMAN SKIN

CHAPTER ONE 1.0 INTRODUCTION  A diverse microbial flora is associated with the skin and mucous membrane of every human being from shortly after birth until death. The human body which contains about 1013 cells routinely harbors about 1014 bacteria. This bacterial population constitutes the normal microbial flora. The normal microbial flora is relatively stable with […]

THE EFFECTS OF PESTICIDES DICHLORODIPHENYL TRICHLOROEYHANE (D.D.Y) AND BENZENE HEXACHRORIDE (BHC) ON THE MICROFLORA OF THE THREE TYPES OF SOIL

ABSTRACT The effect of pesticides, chchlorodipheny trichloroethane (DDY) and Benzene hexaxhoride (BHc) on the  microflora of the three types of soil (loamy, clay and sandy soil) was conducted. The serial dietitian technique was employed up to 10-3 using nutrient agar, potato dextrose agar and sabourand agar plates. The isolation of both bacterial and fungal organisms […]

INVITRO DETERMINATION OF BACTERIOCIDAL EFFECT OF GARLIC ON STAPHYLOCOCCUS AUREUS

ABSTRACT Invitro determination of bacteriocidal effect of garlic extract on staphylococcus aureus causing skin and urinary tract infection (UTI) on some female patients were studied.  High vagina swab (HVS) samples were collected randomly from selected number of patients who had symptoms similar to that of Staphylococcus aureus activities.  The samples were inoculated on Macconkey agar and nutrient agar […]

COMPARATIVE ANALYSIS OF PIPEBORNE WATER AND OTHER SOURCES OF WATER WITHIN ENUGU METROPOLIS

ABSTRACT Comparative analysis  of water was  carried out on well water, stream and pipe born water obviated within Emene locality of Enugu state the sample were subjected to some analytical tests final like presumptive tests, comfirmative test final like presumptive tests, comfirmative test, completed test, indole test and plate count for the organism that develop […]

EFFECT OF STEEPING PERIOD ON YIELD AND ACCEPTABILITY OF STARCH EXTRACTED FROM SORGHUM (SORGHUM BICOLOR, WHITE VARIETY AND RED VARIETY)

ABSTRACT Starch was extracted from two varieties of sorghum bicolor grains (white and red varieties) steeped for 6, 8 and 12 hours by wet milling method, the starch samples were analysed for yield, functional properties and overall acceptability. The yield of the white variety ranged from 0.45 – 0.70kg and the red variety was 0.40 […]

BACTERIA ASSOCIATED WITH URINARY TRACT INFECTION IN PREGNANT WOMEN AND THEIR ANTIBIOTIC SUSCEPTIBILITY

ABSTRACT Urine Sample  from pregnant  women were analysed for bacteriuria. The result of irinalysis reveals the appearance –yellow and Cloudy, pH 6.0, protein-ve, Glucose-ve,Blood-ve, Urobilinogen. Normal, Ketone-ve, Nitrate tve, Bilirubin-ve, Ascobic acid –ve in some of the samples. The result  of urine microscopy reveal pus cells.  4-6/HPF, Epithelial  cell +++, red cells nil, yeast cells […]

THE INCIDENCE OF SALMONELLA AND ESCHERICHIA COLI IN LIVESTOCK (POULTRY) FEEDS

ABSTRACT This work is aimed at determining the incidence of bacterial injections such as Salmonella and Escherichia coliwhich cause health hazards to the  poultry in their feeds.  Sample feed were obtained from commercial mash operation/ pellet mill operation at New heaven and top feed at Ogbete main market Enugu.  The samples were screened for total aerobic bacteria count, […]

THE EFFECT OF MANAGEMENT ON HUMAN DEVELOPMENT

TABLE OF CONTENTS CHAPTER ONE 1.0              Introduction CHAPTER TWO 2.0              Causes and Effect of Malnutrition 2.1       Under Malnutrition 2.2              Malnutrition in Children 2.3              Psychological Effect 2.4              Food Habits 2.5              Taboos and Superstition 2.6              Nutritional Basic Education CHAPTER THREE 3.0              Obesity 3.1       Causes Effect of Obesity 3.2              Metabolic Disorder Associated with Obesity 3.3              Clinical Approach 3.4              Psychological Effect 3.5              Prevention of Obesity CHAPTER FOUR Conclusion References CHAPTER ONE […]

ISOLATION IDENTIFICATION AND CHARACTERIZATION OF FUNGI ASSOCIATED WITH THE SPOILAGE OF CORN

TABLE OF CONTENTS CHAPTER ONE 1.0        Introduction 1.1    Statement of the problem 1.2    Aims/Objective of the Study 1.3    Limitation of the Study 1.4    Significance of the Study CHAPTER TWO 2.0        Literature Review 2.1    Maize as Sweet Corn 2.2    Types of Sugary Corn and Their Qualities 2.2.1 White Corn 2.2.2 Yellow Corn 2.2.3 Bi-Coloured Corn 2.2.4 Mlti-coloured corn […]

MICRO-ORGANISM ASSOCIATED WITH SPOILAGE OF TOMATOES (CANNED TOMATOES)

TABLE OF CONTENT Chapter one 1.0        Introduction Chapter Two 2.0        Literature  review 2.1    History of canned tomato 2.2    Types of canned Tomatoes 2.3    Nutrition facts of canned tomatoes 2.4    Common additions of canned tomato 2.5    causes of canned tomatoes spoilage 2.6    Control/preservation of canned tomato Chapter Three 3.0        Materials  and Methods 3.1    Place of experimental work 3.2    materials […]

BICONTROL POTENTIAL OF BACILLUS THURINGIENSIS ISOLATED FROM SOIL SAMPLES AGAINST LARVA OF MOSQUITO

ABSTRACT A major challenge for achieving successful mosquito control is overcoming insecticide resistance. Bacillus thuringiensis which is one of the most effective biolarvacide for control of species of mosquitoes and monitoring of larval susceptibility is essential to avoid resistance development. Mosquito larvacidal activity of Bacillus thuringiensis was assessed by isolating them from ecologically different soil […]

ANTIBACTERIAL ACTIVITY OF THREE TYPES OF MEDICATED SOAPS OF STARHYROCOCCUS AUREUS FORM WOULD INFECTIONS

ABSTRACT The antibacterial activity of three type of medicate soap on staphylococcus aureus isolated from wound infections was conducted. Fifty individual with would infection within the age range of 9-73 years were sampled. Swabsticks were used to collect specimens from wound infection. each swabstick was streaked separately over plates of nutrient and macconkey agar and […]

SUSCEPTIBLES OF SALMONELLA TYPHI AND OTHER BACTERIAL PATHOGENS TO ANTIBIOTICS AND HOT AQUEOUS EXTRACT OF HIBISCUS SABDARIFFA

ABSTRACT The susceptibilities of Salmonella typhi and other pathogens to antibiotics and hot aqueous extract of Hibiscus sabdariffa were investigated using agar diffusion and agar well diffusion methods respectively. Salmonella typhi was sensitive to ampicillin, cetriaxone, ciprofloxacin, gentamycin of ofloxacin and perfloxacin. Nitrofurantoin, ampicillin, clarithomycin and augumentin are resistant. Escherichia coli, Klebsiella spp, and Staphylococcus […]

PREVALENCE OF ANTIMICROBIAL SUSCEPTIBILITY OF GRAM NEGATIVE BACTERIA IN THE URINE OF CARITAS UNIVERSITY STUDENTS

ABSTRACT In order to access the prevalence and sensitivity pattern of urinary pathogens, 60 midstream urine samples from students of Caritas University were investigated using cultural methods. Samples were examined microscopically and cultured in blood agar and Macckonkey agar. Disk diffusion method was used for antibiotic testing. Of the 60 urine samples 48 yielded significant […]

PRODUCTION AND QUALITY EVALUATION OF BANANA (MUSA SAPIENTUM) WINE

ABSTRACT Juice was extracted from banana (Musa sapientum) pulp with the addition of lemon juice and was inoculated with Baker’s yeast (Saccharomyces cerevisiae) and held at 30 for seven days. The result of the yeast count increases at 48hr, and at 96hr the yeast count decreased gradually. It ranges from 4.9×107 cfu/ml at 0hr, 5.1×107 at the 48hr and 4.8×107 cfu/ml at 168hr. The […]

MICROBIAL PROFILE OF SUYA MEAT

ABSTRACT Ten (10) samples of suya meat in Enugu were collected randomly and analyzed microbiologically and the isolates were identified as Staphylococcus aureus (35%)Escherichia coli (15%) Streptococcus species (15%) Pseudomanas (35%) . The most frequently isolated organism was Staphylococcus and Pseudomonas. The total viable bacterial counts ranged from 1.9x 3.8x cfu/g whereas, total coliform count ranged 1.1x-3.0x cfu/g on MacConkey and Nutrient agar respectively. […]

MICROBIAL EXAMINATION OF SPOILT PERSEA AMERICANA

CHAPTER ONE 1.1   INTRODUCTION The Avocado fruit, Persea americana is a tropical and sub-tropical fruit tree, originated in central America or adjoining regions of North or South America. It has now spread too much of the near tropical world. The avocado is limited especially by its climatic requirements, with race differences. It is also highly susceptible to drought […]

STUDY OF LIPID PROFILE IN STREPTOZOTOCIN (STZ) – INDUCED DIABETIC RATS UNDER NICOTINAMIDE COVERAGE TREATED WITH OCIMUM GRATISSIMUM (O.G.) AND VERNONIA AMYGDALINA (V.A.)

ABSTRACT The effect of leaf extract of ocimum gratissimium (scent leaf) and vernonia Amygdalina (bitter leaf) under nicotinanmide coverage was studied using albino wistar rat. Acute toxicity of the plant was carried out using (Lorkes method). LD50 was calculated to be 208 mglkg. The main work involved 49 albino wistar rats weighing 150g to 200g. […]

A STUDY ON MOSQUITO AS A PRIMARY MALARIA VECTOR

CHAPTER ONE 1.1     INTRODUCTION  Mosquitoes are vectors of Plasmodium parasite that cause malaria, nematode worms that cause filariasis for example Wuchereria bancrofti, and a large number of arbo-viruses e.g yellow fever and dengue viruses including two of great impacts in the tropical ad sub-tropical regions (Ifeyinwa et al, 2012). They belong to the Order:Diptera and possess short […]

AN ANALYSIS ON THE EFFECT OF Hippocratea africana ROOT BACK EXTRACT ON ESTRADIOL CONCENTRATION IN FEMALE ALBINO WISTAR RATS

CHAPTER ONE 1.0   INTRODUCTION Malaria is one of the most important killer diseases in Africa causing more than one million deaths every year. In Nigeria, the infection rate has been described as holoedenic with more than 75% of children ages 2-9 years infected (Saloko et al., 1994). Persistence of malaria symptoms after treatment with over the counter […]

EVALUATION OF THE ANTIHYPERGLYCEAMIC EFFECTS OF POLAR PARTITION FRACTIONS OF HOSLUNDIA OPPOSITA LEAF

ABSTRACT Hoslundia opposita leaf is used folklorically in the management of diabetes and a preliminary anti-hyperglycaemic activity has been reported for its methanol extract. Therefore, in this study, the antihyperglyceamic activities of the partition fractions of Hoslundia opposita leaf was investigated using glucose loaded wistar rats with a view to determine the most active fraction. […]

AETIOLOGICAL AGENTS OF THE WOUND INFECTIONS AND THEIR ANTIMICROBIAL SUSCEPTIBILITY PATTERN

INTRODUCTION The primary function of intact skin is to control microbial population that live on the skin surface and to prevent underlying tissue from becoming colonized and invaded by potential pathogens (Ndipet. al., 2007). Exposure of subcutaneous tissue following a loss of skin integrity (i.e. wound) provides a moist, warm and nutritious environment that is conducive […]

AMYLOLYTIC AND BETA-GLUCANASE ACTIVITIES OF YAM ROT FUNGI

ABSTRACT Yam popularly called “Ji” in Igbo Language is a tropical crop belonging to the Family Dioscoreaceae in the genus Dioscorea. It has as many as 600 species out of which six are economically important staple species. These are: Dioscorea rotundata (white guinea yam),Dioscorea alata (water yam), Dioscorea bulbifera (aerial yam), Dioscorea esculant (Chinese yam) and Dioscorea dumetorum (trifoliate yam). Out of these, Dioscorea rotundata (white yam) andDioscorea […]

BACTERIOLOGICAL STUDY OF SACHET WATER

ABSTRACT Water is one of the indispensable resources for the continued existence of all living things including man. The provision of an adequate supply of safe drinking water was one of the eight components of primary health care identified by the International Conference on Primary Health care in 1978. This study investigated the bacteriological quality […]

BACTERIOLOGICAL STUDY OF WOUND

ABSTRACT The majority of dermal wounds are colonized by anaerobic microorganisms that originate predominately from mucosal surfaces such as those of oral cavity and gut. The analysis was carried out in the Microbiology Laboratory, Imo state University for the bacteriology study of wound. 10 Samples were collected from Federal Medical Center (FMC), Owerri, Imo State. […]

BIOPHYSICAL PROPERTIES OF WASTE WATER FROM FISH POND

ABSTRACT Agriculture in the early days was generally considered too small an industry to have significant impact on the environment. The remarkable growth of the agriculture industry in many countries over the past decades has increased adverse impact on the environment. (Acketors, 2014). The cultivation of organisms in ponds (Tincker, 2012), tanks (Millamena et al., 1991), rivers […]

COMPARISON OF ANTI-DANDRUFF ACTIVITY OF SYNTHETIC SHAMPOOS AND CRUDE PLANT EXTRACTS ON DANDRUFF CAUSING ISOLATE

ABSTRACT Dandruff is a scalp disorder which is characterized by excessive shedding of skin cells from the scalp. It is a common problem faced by people of all age groups. As the epidermal layer continually replaces itself, cells are pushed outward where they eventually die and flake off. For most individuals, these flakes of skin are too […]

DETERMINATION OF MICROBIAL LOAD ON SMOKED FISH SOLD

ABSTRACT Microbial assessment of smoked Scombia scombia spp. of fish procured from two markets in Owerri metropolis was carried out. The samples were analyzed using bacteriological and fungi media. The average bacteria counts ranges from 3.1X106-6.8X106cfu/g while the average fungi counts ranges from 0.0-0.3X106cfu/g. Four species of bacteria were isolated which areStaphylococcus aureus, Escherichia coli, bacillus spp., […]

EFFECT ON CASSAVA MILL EFFLUENT IN THE SOIL

ABSTRACT The microbiological  and physiochemical characteristics of soil receiving cassava effluent in Orji, Imo State, Nigeria were carried out, soil samples were collected from site polluted with cassava mill effluent and also from adjacent site not polluted with cassava effluent to serve as control. The overall population of microorganisms in cassava mill effluent was determined. Isolation […]

EXAMINATION OF SOME LOCALLY FERMENTED FOODS FOR PROBIOTICS BACILLUS COAGULANS

ABSTRACT Probiotics are defined as live microorganisms that, when administered in adequate amounts, confer a health benefit on the host (FAO/WHO, 2001). Probiotics is a general name for microorganisms that are associated with beneficial effects for humans and animals. They contribute to intestinal microbial balance and play a role in maintaining health (Soccol et al., 2010). […]

FUNGI ASSOCIATED WITH SPOILAGE OF SMOKED DRIED FISH

ABSTRACT Fish is highly nutritious with high protein content. However, it is a suitable medium for growth of microorganisms, if poorly processed (Oparaku and Mgbenka, 2012). The growth of microorganisms and other non-microbial activities such as lipid oxidation contribute to the deterioration of fish products (Martin, 2010). An increase in the ambient temperature triggers favourable […]

THE EFFECTS OF MATERNAL NUTRITION ON THE EXCLUSIVE BREASTFEEDING OF INFANTS IN EGOR LOCAL GOVERNMENT AREA OF EDO STATE

ABSTRACT The researcher investigated the effects of maternal nutrition on the exclusive breastfeeding of infants in Egor Local Government Area of Edo State. The purpose of the study was to find out the effects of maternal nutrition, socio-economic status, occupation, and perception on the exclusive breastfeeding of infants in Egor Local Government Area. To guide the […]

THE ANTIMICROBIAL ACTIVITY OF THE CRUDE METHANOL EXTRACT AND FRACTIONS OF ALBIZIAZYGIA STEM- BARK

CHAPTER ONE 1.0 INTRODUCTION Plants had been used for medicinal purposes long before recorded history. Ancient Chinese and Egyptian papyrus writings describe medicinal uses for plants as early as 3000BC. Indigenous cultures(such as African and Native Americans) used herbs in their healing rituals, while others developed traditional medical systems in which herbal therapies were used. […]

ASSESSMENT OF THE EXISTING DUMPSITES

CHAPTER ONE Introduction Background of Study Wastes can be considered, as those materials no longer required by an individual, institution or industry. Wastes are thus regarded as by-products or end products of the production and consumption process respectively. Solid waste can be defined as: ‘The useless and unwanted products in the solid state derived from […]

RELEVANCE OF BLOOD CULTURE TO THE DIAGNOSIS AND TREATMENT OF SEPTICAEMIA

Chapter one 1.0   Introduction 1.1   Blood culture 1.2   Septicaemia 1.3   Objectives of the research 1.4   Justification Chapter two 2.0     Literature review 2.1     Septicaemia 2.2     Causes of septicaemia 2.3     Detecting bacteria from the blood in septicaemia 2.4     Factors that predispose to bloodstream infection 2.5     Causative organisms 2.6     Blood culture in septicaemia (sepsis) 2.7     Diagnosis of […]

MICROORGANISM ASSOCIATED WITH SPOILAGE OF TOMATOES (CANNED TOMATOES)

TABLE OF CONTENT Chapter one 1.0        Introduction Chapter Two 2.0        Literature  review 2.1    History of canned tomato 2.2    Types of canned Tomatoes 2.3    Nutrition facts of canned tomatoes 2.4    Common additions of canned tomato 2.5    causes of canned tomatoes spoilage 2.6    Control/preservation of canned tomato Chapter Three 3.0        Materials  and Methods 3.1    Place of experimental work 3.2    materials […]

THE INCIDENCE OF SALMONELLA AND ESCHERCHIA COLI IN LIVESTOCK (POULTRY) FEEDS

ABSTRACT This work is aimed at determining the incidence of bacterial injections such as Salmonella and Escherichia coli which cause health hazards to the  poultry in their feeds.  Sample feed were obtained from commercial mash operation/ pellet mill operation at New heaven and top feed at Ogbete main market Enugu.  The samples were screened for total aerobic bacteria count, […]

EVALUATING THE IN-VIVO EFFECTS OF METHANOLIC EXTRACT OF A. BOONEI ON LIVER ENZYME FUNCTION IN ALLOXAN INDUCED DIABETES MELLITUS.

INTRODUCTION 1.1Background of Study The present decade has witnessed a great and intense resurgence in the interest and use of the plant (Briskin., 2000). The healing power of herbs identified and botanical medicine has been one of the oldest practiced professions by humanity (Oduolaet al ., 2007). In fact, the use of synthetic pharmaceutical products […]

MICROBIOLOGICAL EVALUATION OF SELECTED CREAMS

ABSTRACT Cosmetic(creams) need not be sterile,however they must not be unduly contaminated with micro-organism and should remain in a stable state throughout the shelf life of the product (or when be used by the consumer).the aim of this project was to determine the microbial load in selected creams and to identify the specific contaminants. For […]

COMPARATIVE STUDY OF ANTIBACTERIAL ACTIVITY OF TWO SELECTED MEDICATED SOAP AND ONE LOCAL BLACK SOAP ON STAPHYLOCOCCUS AUREUS FROM WOUND INFECTION

ABSTRACT Two medicated soaps and one local soap were investigated for their antibacterial activities against Staphylococcus aureus. Identification of the bacteria species was by standard microbiological techniques which included colonial examination, gram staining, and biochemical testing. Minimum inhibitory and minimum bactericidal activities of the soaps were determined by disc agar diffusion method. Black soap has the […]

EVALUATION OF ELEPHANT GRASS (PENNISETUM PURPUREUM) AS SUBSTRATE FOR BIOETHANOL PRODUCTION USING CO-CULTURES OF ASPERGILLUS NIGER AND SACCHAROMYCES CEREVISIAE

ABSTRACT Elephant grass (Pennisetum purpureum) was evaluated for its ethanol production potential using co-cultures of Aspergillus niger and Saccharomyces cerevisiaeisolated from local sources. Proximate and lignocellulose analysis carried out on the plant sample showed that it had crude fibre, lignin, hemicellulose and cellulose contents of 31.5%, 26.78%, 18.76% and 34.16% respectively. Aspergillus niger strains were isolated from soil and bread […]

SYNERGISTIC EFFECT OF BACTERIOCIN-LIKE INHIBITORY SUBSTANCES PRODUCED BY VAGINAL Lactobacillus AGAINST GROUP B Streptococci

ABSTRACT The vaginal normal flora of a healthy woman is dominated by species of Lactobacillus. The balance between the vaginal flora is maintained by the protective antagonizing compounds produced by Lactobacillus. Group B Streptococci (GBS) is an opportunistic organism usually carried asymptomatically by pregnant women but cause severe neonatal infections. Antibiotics chemotherapy poses a lot of threat especially to pregnant women. […]

STUDIES ON THE OCCURRENCE OF BETA-LACTAMASES IN MEMBERS OF THE GENERA Salmonella AND Shigella ISOLATED FROM VARIOUS HOSPITALS

ABSTRACT Salmonella and Shigella species isolated from human faecal samples were examined forBeta-lactamase production and resistance to some antibiotic agents. Age distribution of sources of resistant isolates showed thatShigella isolates from Youths (18 – 30 years) were more resistant to Ampicillin (66.67%) and Augmentin (33.33%) than isolates from Infants (6 months – 4 years) which showed percentage resistance of […]

STUDIES ON THE CO-INFECTIVITY OF HIV AND ATYPICAL MYCOBACTERIA

ABSTRACT The increasing global incidence of tuberculosis and nontuberculous mycobacteria (NTM) has been attributed to immunosuppresion due to the Human immunodeficiency virus (HIV) epidemic.  The paucity of information on the contribution of NTM to mycobacterial infections in Africa including Nigeria has however been closely associated with limited laboratory culture for its isolation and identification. This study […]

MULTIDRUG RESISTANCE PROFILES OF CLINICAL AND ENVIRONMENTAL ISOLATES OF PSEUDOMONAS AERUGINOSA AND ESCHERICHIA COLI

Abstract The emergence of multiple antibiotic resistance in bacteria and the indiscriminate use of antibiotics contribute to the dissemination of resistant pathogens in the environment which may cause problems in therapy and is a serious public health issue. This study was conducted to determine the incidence of Pseudomonas aeruginosa and E.coli isolates in certain clinical and environmental samples as […]

IDENTIFICATION AND EVALUATION OF IMMUNO-MODULATORY PROPERTIES OF PREBIOTICS EXTRACTED FROM Vernonia amygdalina

ABSTRACT Prebiotics are non-digestible food substances that when consumed provide a beneficial physiological effect on the host, by selectively stimulating the favourable growth or activity of a limited number of indigenous bacteria. Most known prebiotics are complex carbohydrates commonly found in fruits, vegetables and plant products. Vernonia amygdalina, commonly called bitter leaf is widely consumed in […]

ANTIMICROBIAL ACTIVITIES AND PHYSICO-CHEMICAL ANALYSES OF HONEYS FROM Hypotrigona sp., Melipona sp. and Apis mellifera (BEE HONEY)

ABSTRACT Honey has been used traditionally for ages to treat infectious diseases. Antimicrobial activity of honey is complex due to the involvement of multiple bioactive compounds. The physico-chemical and antimicrobial properties of honey varieties from Apis mellifera and stingless bees,Hypotrigona sp. (Okotobo – Igbo) and Melipona sp.(Ifufu – Igbo) were studied using International Honey Commission protocols and microbiological methods (agar-well diffusion […]

A COMPARATIVE STUDY OF THE SEROLOGICAL STATUS FOR NEWCASTLE DISEASE IN LOCAL CHICKENS OF LIVE BIRD MARKETS AND HOUSEHOLDS

ABSTRACT Newcastle disease (ND) is the most important viral disease of poultry in the world including developing countries. In Africa and Asia, it is a major constraint against the development of both Industrial and village poultry production. A serological study was conducted to estimate the level of circulating antibodies against ND in unvaccinated local chickens, […]

PURIFICATION AND PROPERTIES OF ALPHA AMYLASE PRODUCED FROM BACILLUS SUBTILIS ISOLATED FROM THE SOIL

ABSTRACT Thermophiles are organisms which are adapted to live at high temperatures. The enzymes from thermophiles find a number of commercial applications because of their thermostability and thermoactivity including in beer brewing. One of the most attractive attributes of thermophiles is that they produce enzymes capable of catalysing biochemical reactions at temperatures higher than those […]

EFFECT OF DIFFERENT CARBON SOURCES ON THE GROWTH OF ANTIMICROBIAL PRODUCING (Bacillus Species IN Citrillus vugaris)

ABSTRACT Effect of different carbon sources on the growth of antimicrobial producing Bacillus species in ogiri-egusi sold in Ogbeta, Orie, Artisian, Kenyatta, Enugu State were evaluated using standard microbiological and analytical methods. 12 bacteria were isolated and identified such as Bacillussubtilis,(33.33), Bacillus pumulis (41.66), Bacillus licheniformis (25) were identified based on their colonybiochemical characteristics.The isolates of the highest […]

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