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PREVALENCE AND ANTIMICROBIAL SUSCEPTIBILITY OF GRAM NEGATIVE BACTERIA IN THE URINE OF UNIVERSITY STUDENTS

ABSTRACT In order to access the prevalence and sensitivity pattern of urinary pathogens, 60 midstream urine samples from students of Caritas University were investigated using cultural methods. Samples were examined microscopically and cultured in blood agar and Macckonkey agar. Disk diffusion method was used for antibiotic testing. Of the 60 urine samples 48 yielded significant […]

ISOLATION OF MICROCOCCUS FROM FERMENTED UGBA

TABLE OF CONTENT Title page Certification Dedication Acknowledgement Table of content CHAPTER ONE 1.0    Introduction 1.1    Aims and objectives CHAPTER TWO Literature review 2.1    Fermented foods 2.2    Pentaclethra macrophylla 2.3    Botany 2.4    Ecology 2.5    Growt and Development 2.6    Method of production 2.7    Micrococcus species in ugba 2.8    Environmental isolation of micrococcus CHAPTER THREE 3.0    Materials […]

INVESTIGATING THE LEVEL OF MICROBIAL CONTAMINATION OF YOGHURTS SOLD

CHAPTER ONE 1.0   INTRODUCTION Yoghurt is a cultural diary product produced by lactic fermentation of milk (Hui, 1992). Yoghurt is produced by the controlled fermentation of milk by two species of bacteria; Lactobacillus bulgaricus and Strptococcus thermophilus.         These two species of bacteriae have now been established as the yoghurt starters. Any sort of milk may be used to make yoghurt, but […]

DETERMINING THE MAJOR AETIOLOGIC AGENT OF URETHRITIS

CHAPTER ONE 1.0   INTRODUCTION Chlamydiae are small gram-negative obligate intracellular microorganisms that preferentially infect squamocolumnar epithelial cell (www. Google. Com.2010).         Chlamydia is the most prevalent sexually transmitted disease resulting in urethritis, with frequencies exceeding those of Neisseria gonorrhoeae         In the United State for instance, roughly four million cases are reported annually mostly occurring in men and women […]

DETERMINING THE CULTURE CONDITION FOR MAXIMUM LIPASE PRODUCTION BY ISOLATED MICROCOCCUS FROM UGBA

CHAPTER ONE 1.0   INTRODUCTION         Ugba also called ukpaka is a popular food delicacy in Nigeria especially among Ibo ethnic group. It is rich in protein and is obtained by a solid state fermentation of the seed of African oil bean tree (Pentaclethra macrophylla Benth). It is essential food item from various traditional ceremonies where it is mixed […]

COMPARATIVE STUDY OF DISINFECTANT EFFICIENCY OF ETHANOL, BLEACH AND PHENOLICS AGAINST Pseudomonas aeruginosa and Staphylococcus aureus

ABSTRACT Ethanol, Bleach and Phenolics are three kinds of disinfectants which have been widely used in common laboratories.  In this study, a compared experiment on these three disinfectants efficiency was conducted against Staphylococcus aureus and Pseudomonas aeruginosa using agar hole diffusion method. Different concentrations of bleach (1%, 2%, 3%, 4% and 5%) were used on both organisms.  Also (50%, 60%, […]

ASSESSMENT OF NEWCASTLE DISEASE VIRUS ANTIBODIES AMONG BIRDS

CHAPTER ONE 2.0                         INTRODUCTION Newcastle disease (ND) is one of the most important avian viral diseases because of its high economic impact on the poultry industry. Newcastle disease was discovered in Newcastle upon Tyne. Newcastle upon Tyne is a city and a metropolitan borough of Tyne and wear, in northeast England in 1926 (Doyle, 1927), but at […]

THE BACHERIOLOGICAL EXAMINATION OF IDODO RIVER

ABSTRACT This work was aimed at assessing microbial coal of Idodo river in Enugu state Nigeria.  The samples of river was obtained from the arc of low activity, and a place where human activities are very high.  The samples were collected very early in the morning and afternoon.  They were examined to determined the bacterial organisms that are […]

COMPARATIVE ANALYSIS OF PIPEBORNE WATER AND OTHER SOURCES OF WATER

ABSTRACT Comparative analysis  of water was  carried out on well water, stream and pipe born water obviated within Emene locality of Enugu state the sample were subjected to some analytical tests final like presumptive tests, comfirmative test final like presumptive tests, comfirmative test, completed test, indole test and plate count for the organism that develop on  the culture, […]

THE INCIDENCE OF SALMONELLA AND ESCHERIDA COLI ON POULTRY AND THEIR FEEDS

CHAPTER ONE 2.0     INTRODUCTION The term poultry used in agriculture generally refers to all domesticated birds kept for egg or meat production.  These includes chickens (domestic fowls),  Grallus domestics, turkeys, ducks and geese.  This project will deal largely with the chicken which are the most common domestic fowl.  The species of poultry are habitable or can survive in different environments […]

PRODUCTION OF PROTEASE BY ASPERGILLUS FLAVUS IN SOLID STATE FERMENTATION

ABSTRACT The study for the production of protease from Aspergillus flavus using wheat bran as substrate under Solid State Fermentation was conducted in University of Abuja, Department of Microbiology. Aspergillus flavus was isolated from spoilt bread and was identified on the basis of the morphological assessment such as macroscopic and microscopy. Among the characteristics used includes: colonial characteristics such […]

MYCOFLORA OF STORED LOCUS BEANS (PARKIA BIGLOBOSA) PURCHASED FROM USELU MARKET

ABSTRACT Stored locust bean samples were purchased from Uselu market and analysed for their fungal load and physicochemical properties such as moisture content, pH value and titratable acidity. Enumeration of the total fungal species was carried out by pour plate technique using appropriate medium, potato dextrose agar (PDA) with antimicrobial agents (streptomycin), incorporated to inhibit […]

ISOLATION, IDENTIFICATION AND ANTIBIOGRAM OF STAPHYLOCOCCUS AUREUS ISOLATED FROM COW MEAT

Abstract The study was carried out with aim to isolate Staphylococcus aureus from cow meat and determine the antibiogram pattern of S. aureus. Three samples of cow meat from three different locations (Gwagwalada market, Abattoir market and Kasuan Dare) in Gwagwalada were collected to isolate S. aureus from the samples. The organisms isolated were Salmonella spp, Escherichia coli, Staphylococcus aureus and Listeria monocytogenes. Several biochemical tests were […]

ISOLATION OF MICRO-ORGANISMS FROM STORED PAP

CHAPTER ONE INTRODUCTION 1.1     BACKGROUND OF THE STUDY Cereals have been known to man from the earliest times porridge prepared from cereals are  eaten in different parts o the world, especially in developing countries where they may present the basic diet.  This porridge could be baked to enhance the taste, quality and improve digestibility (Oke 1967, Adeniyi and […]

ISOLATION AND CHARACTERIZATION OF FUNGI ASSOCIATED WITH “SPOILED” TOMATOES

ABSTRACT The study examined the various fungal pathogens associated with tomatoes spoilage as well as their preliminary taxonomic grouping up to genus level. 25 samples of the spoiled tomatoes were collected from five locations within Gwagwalada area council. Locations include: Passo Village, Gwagwalada market, Phase III, New Kutunku and Dagiri. Thesame prevalence rate(100%) was recorded […]

DETERMINATION OF THE MASS TRANSFER CHARACTERISTIC OF THERMAL DRYING AND OSMOTIC DEHYDRATION OF PUMPKIN AND BITTER LEAF

ABSTRACT The experiment was carried out  and three osmotic solutions were prepared, “hypertonic solution 60% concentrated, hypotonic solution 40% concentrated and isotonic solution 50% concentrated” and oven drying was also carried in the course of the experiment, the effect of sodium chloride (osmotic agent) concentration, temperature and immersion time on overall mass transfer coefficient, effective diffusivity, […]

DETERMINATION OF DEGRADING ABILITY OF FUNGI ISOLATED FROM HYDROCARBON POLLUTED SOIL ON CRUDE OIL USING GAS CHROMATOGRAPHY

CHAPTER ONE 1.1INTRODUCTION Crude oils are composed of mixtures of paraffin, alicylic and aromatic hydrocarbons. Microbial communities exposed to hydrocarbons become adapted, exhibiting selective enrichment and genetic changes resulting in increased proportions of hydrocarbon-degrading bacteria and bacterial plasmids encoding hydrocarbon catabolic genes (Leahy and Colwell, 2004). Adapted microbial communities have higher proportions of hydrocarbon degraders […]

COMPARATIVE ANALYSIS OF ANTIMICROBIAL STRENGTH OF THREE MOST COMMONLY USED ANTIBIOTICS

ABSTRACT In comparing the antimicrobial strength of three most common antibiotics, which includes: streptomycin chloranphenicol and gentarccin. Samples of urine and high vaginal swab (H.V.S) were collected from park lane Enugu. The organisms isolated were pure culture of staphylococcus aureus and Escherichia coli Sensitivity test was carried out with  the use of sensitivity disk containing various […]

BACTERIOLOGICAL QUALITY OF FROZEN CHICKEN

ABSTRACT Twenty samples of frozen chicken from five different sellers were analyzed to determine their bacteriological load; the samples were collected from Gwagwalada market. All the frozen chicken samples from the five sellers examined were contaminated with some bacterial species namely, Bacillus cereus, Escherichia coli, Staphyloccus aureus, Staphylococcus epidermidis, Proteus vulgaris, Klebsiella pneumonia, Pseudomonas aeruginosa. The total […]

BACTERIOLOGICAL ANALYSIS OF WATER TANKS

ABSTRACT An investigative study was carried out to determine the bacteriological qualities of borehole water samples in halls of residence in the University of Benin, Ugbowo Benin City. A total of five water samples were collected from the the various halls of residence in the school. Bacteriological analysis was carried out using standard methods. The […]

ASSESSMENT OF NUNU PRODUCED USING PURE STARTER CULTURE

ABSTRACT Nunu is Nigerian locally fermented milk product commonly prepared by Hausa/Fulani cattle rearers. It’s mostly available in the Northern part of Nigeria. It’s production and consumption derives food security and economic benefits to the rural people in the region. However, the process characteristics results in products which are not appealing to many people. Lactic […]

ASSESSMENT OF MICRONUTRIENTS IN CHILDREN WITH MALARIA INFECTION

ABSTRACT The increased clinical state of malaria infection may be due to poor nutritional status most especially as a result of micronutrients deficiency. Micronutrients play vital role both in combating anaemia and other adverse effects of malaria infection in humans and animals in developing resistance against the disease. This research was aimed at establishing the effect of malaria […]

A SURVEY OF THE ANTIBACTERIAL ACTIVITY OF THYMUS VULGARIS AND MYRISTICA FRAGRANS ON STREPTOCOCUSS PNEUMONIAE AND PSEUDOMONAS AERUGINOSA

ABSTRACT The aqueous and ethanolic extracts of the leaves of Thymus vulgaris (thyme) and the seeds of Myristica fragrans (nutmeg) were screened for their antibacterial activity and phytochemical constituents. The standard methods used to detect the phytochemicals present in the extracts revealed the presence of tannins, flavonoids, triterpenoids and phytosterols in all the extracts while steroids and amino acids […]

THE PREVALENCE OF CHLAMYDIA INFECTION

CHAPTER ONE INTRODUCTION Chlamydiae are small gram-negative obligate intracellular microorganisms that preferentially infect squamocolumnar epithelial cell (www. Google. Com.2010). Chlamydia is the most prevalent sexually transmitted disease resulting in urethritis, with frequencies exceeding those of Neisseria gonorrhoeae In the United State for instance, roughly four million cases are reported annually mostly occurring in men and women […]

THE LEVEL OF MICROBIAL CONTAMINATION OF YOGHURTS

CHAPTER ONE INTRODUCTION    Yoghurt is a cultural diary product produced by lactic fermentation of milk (Hui, 1992). Yoghurt is produced by the controlled fermentation of milk by two species of bacteria; Lactobacillus bulgaricus and Strptococcus thermophilus.         These two species of bacteriae have now been established as the yoghurt starters. Any sort of milk may be used to make […]

MICROBIAL CONTAMINATION OF YOGHURT

CHAPTER ONE 1.0   INTRODUCTION Yoghurt is a fermented dairy product obtained from the lactic acid fermentation of milk. It is one of the most popular fermented milk products in the world and produced commercially at home. (Willey et al., 2008). In its commercial production, non fat or low fat milk is pasteurized cooled to 43°c and are inoculated […]

ANTIMICROBIAL PROPERTIES OF CHLORINE AND ALCOHOL DISINFECTANTS

INTRODUCTION 1.1 Background of the study Antiseptics and disinfectants are used extensively in hospitals and other health care settings for a variety of topical and hard-surface applications. In particular, they are an essential part of infection control practices and aid in the prevention of nosocomial infections. Mounting concerns over the potential for microbial contamination and infection […]

ANTIBIOTICS RESISTANCE PROFILE OF ESCHERICHIA COLI ISOLATED FROM APPARENTLY HEALTHY DOMESTIC LIVESTOCK

ABSTRACT This study was conducted to determine the antibiotic resistance profile of Escherichial Coli isolate from apparently healthy domestic livestock viz: cow, goats and chicken from Osun State Nigeria. E. Coli was isolated using Eosin methylene Blue Agar (EMB) and identified by conventional microbiological technique. The isolate were tested against 14 antibiotics using the disc […]

KNOWLEDGE, ATTITUDE AND PERCEPTION OF LASSA FEVER AMONG PEOPLE ATTENDING PHC

CHAPTER ONE INTRODUCTION 1.1     Background of Study Lassa fever is one of the diseases for which weekly epidemiological reporting to the health authorities is being done in Nigeria. A rapidly changing epidemiological pattern had been reported over the years (Ogbu, Ajuluchukwu and Uneke, 2007). It causes mortality and morbidity where outbreaks occur worldwide including Nigeria […]

KNOWLEDGE AND PRACTICE OF HAND WASHING AS A MEASURE OF DISEASE CONTROL AMONG STUDENTS IN AFE BABALOLA UNIVERSITY

ABSTRACT The purpose of the thesis was to study the knowledge and practice of hand washing as a measure of disease control among students in Afe Babalola University. The goal was to know how well the knowledge of hand hygiene is known to the students and their attitudes towards maintaining good hand hygiene and to […]

KNOWLEDGE, AWARENESS AND PERCEPTIONS OF CORONAVIRUS IN NIGERIA

Abstract This study was carried out on the knowledge, awareness and perceptions Covid 19 pandemic in Nigeria. Mass gatherings at sporting and religious events attract huge crowds, creating high-risk conditions for the rapid spread of infectious diseases. The study was carried out in Ibadan, Oyo state. During the first week of March, the surge of […]

KNOWLEDGE, ATTITUDE AND PERCEPTION OF COVID-19 PANDEMIC AMONG RESIDENTS OF THREE COMMUNITIES OVIA-NORTH EAST, EDO STATE

Abstract This study was carried out on the knowledge, attitude and perception of Covid 19 pandemic in Nigeria. Mass gatherings at sporting and religious events attract huge crowds, creating high-risk conditions for the rapid spread of infectious diseases.  The study was carried out in Ovia-North East LGA, Edo state. During the first week of March, […]

ISOLATION AND IDENTIFICATION OF BACTERIAL FROM NAIRA NOTE

ABSTRACT Survey of the currency bills in University of Uyo, for fungal contaminations revealed presence of fungal organisms such as species of Aspergillius niger, Aspergillus fumigatus, penicillium spp, mucor spp, candida albicans,and Rhizopus spp. This study was carried out using a total of 101 samples of banknotes denomination (N5, N10, N20, N50, N100, N200, N500, […]

HEALTHCARE IMPACT OF COVID-19 EPIDEMIC IN NIGERIA: A STOCHASTIC MATHEMATICAL MODEL

ABSTRACT COVID-19, since its discovery in 2019, has posed a major health problem in the world. It is caused by the SARS-CoV-2 virus and is transmitted via infected respiratory droplets and contaminated surfaces. There is an urgent need to understand the transmission characteristics of the virus in response to social interventions. This is important to […]

PRODUCTION OF ETHANOL FROM CASSAVA PEELS USING Zymomonas mobilis and Saccharomyces cerevisiae

ABSTRACT The yield of bioethanol from cassava peels hydrolysed with Aspergillus niger and crude enzymes (amylase, cellulase and pectinase) was examined in this study. Cassava peels were pretreated by soaking and a combination of soaking and boiling which removes 67% of cyanide. After hydrolysis, Zymomonas mobilis and Saccharomyces cerevisiae were used to ferment the hydrolysates […]

BACTERIOLOGICAL EXAMINATION OF IDODO RIVER

Abstract This work was aimed at assessing microbial coal of Idodo river in Enugu state Nigeria. The samples of river was obtained from the arc of low activity, and a place where human activities are very high. The samples were collected very early in the morning and afternoon. They were examined to determined the bacterial […]

BACTERIAL CONTAMINANTS ASSOCIATED WITH COMMERCIAL POULTRY FEED FROM THREE DIFFERENT COMPANIES

ABSTRACT  Bacterial contaminants associated with commercial poultry feeds from three different companies in Enugu (Ave investment, Phinomar and Bora feed producing company) were studied. Four types of poultry feeds which include starter, grower, finisher and layer were examined using pour plate techniques. The culture media used were cled agar and salmonella – shigella agar (SSA). […]

BACTERIA RESPONSIBLE FOR UPPER URINARY TRACT INFECTION

BACTERIA RESPONSIBLE FOR UPPER URINARY TRACT INFECTION IN 5 CHILDREN ATTENDING GOVERNMENT SECONDARY SCHOOL MAKARFI  CHAPTER ONE  INTRODUCTION 1.1. Introduction In biology/ecology, parasitism is a non-mutual symbiotic relationship between species, where one species, the parasite, benefits at the expense of the other, the host. Traditionally parasite (in biological usage) referred primarily to organisms visible to […]

BACTERIA AND FUNGI INVOLVED IN YOGURT PRODUCTION

ABSTRACT Yogurt sold at different manufacturer, in Ilorin Kwara State was taken to the laboratory and examined, different bacteria and fungi were found in all the yoghurt tested using conventional methods. The implications of these micro organisms were discussed.           INTRODUCTION AND LITRERATURE REVIEW Yogurt is a popular and healthy snacks […]

AUTOMATION IN INVENTORY MANAGEMENT IN THE PHARMACEUTICAL UNIT OF THE HOSPITAL

CHAPTER ONE INTRODUCTION 1.0 Introduction A pharmaceutical inventory management system is a computerized system that facilitates the inventory management of pharmaceutical products. An automated inventory management system can be categorized as an aspect of computer field of management information system (MIS) and it cannot exist without a proper database management which remains one of the […]

ASSESSMENT OF PATIENT ATTITUDE AND PERCEPTION TOWARDS XRAY EXAMINATION

ABSTRACT The use of X-rays in medical diagnostic radiology has increased globally. The medical use of X-ray for diagnosis of illness is subject to the principles of justification and optimization for the protection of exposed individuals. Medical X-ray accounts for the largest exposure of humans to man-made ionizing radiation. Exposure of individuals can lead to […]

HEMOLYTIC ACTIVITY AND STREPTOMYCIN SUSCEPTIBILITY PROFILE OF BACTERIA ISOLATES ASSOCIATED WITH NASAL SECRETION

TOPIC: HEMOLYTIC ACTIVITY AND STREPTOMYCIN SUSCEPTIBILITY PROFILE OF BACTERIA ISOLATES ASSOCIATED WITH NASAL SECRETION ABSTRACT This study was carried out on hemolytic activity and streptomycin susceptibility profile of bacteria isolates associated with nasal secretion. To date, very little work has been done on the respiratory system flora of apparently healthy cattle. The objective of this […]

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