COMPLETE SCHOOL PROJECT TOPICS & MATERIALS :
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Abstract

This study investigated solvent production by indigenous Clostridium species isolated from soil environments in the Jos metropolis. Soil samples from various locations were analyzed for temperature, moisture content, and pH. After enrichment and isolation, 58 isolates were identified morphologically, with 10 suspected as Clostridium species. Further biochemical tests confirmed Clostridium acetobutylicum and Clostridium auranbutyricum as non-pathogenic. Pathogenic species included Clostridium colinum, Clostridium perfringens, Clostridium botulinum, and Clostridium difficile, with Clostridium botulinum being the most prevalent. Waste disposal sites and nursery gardens showed the highest occurrence of Clostridium species. Ten isolates produced alcohol during fermentation.